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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: LncRNA LINC00662 promotes colon cancer tumor growth and metastasis by competitively binding with miR-340-5p to regulate CLDN8/IL22 co-expression and activating ERK signaling pathway

Fig. 5

miR-340-5p, as a target miRNA of LINC00662, regulated the viability of colon cancer cells (a) Starbase v2.0 database showed that binding site of LINC00662 and miR-340-5p; (b) Luciferase reporter assays were used to prove that miR-340-5p can target LINC00662; (c) Biotin-coupled sense or antisense DNA probes targeting LINC00662 were incubated with HCT29, LS174T, LOVO, and CT26 cells lysate to pull down RNAs, followed by RT–PCR analysis of the amounts of LINC00202 and miR-3619-5p; (d) Biotin-labeled LINC00662 RNA and antisense RNA were incubated with HCT29, LS174T, LOVO, and CT26 cells lysate to pull down RNAs, and subsequently RT–PCR was performed to analyze the miR-340-5p amount; (e) miR-340-5p mRNA level was analyzed by RT-PCR in LINC00662 overexpression plasmids transfected HCT29 and LS174T cells; (f) miR-340-5p mRNA level was analyzed by RT-PCR in LINC00662 knockdown plasmids transfected LOVO and CT26 cells; (g) miR-340-5p mRNA level was analyzed by RT-PCR in colon cancer cell lines; (f) RT-PCR assay was used to detect miR-340-5p mRNA level in colon cancer tissues; (h) Correlation analysis of LINC00662 and miR-340-5p; (i) the transfection efficiency of miR-340-5p inhibitors was analyzed by RT-PCR in HCT29, LS174T, LOVO and CT26 cells; (j and k) CCK8 assay was used to detect cell viability of miR-340-5p inhibitors transfected HCT29 and LS174T cells; (l and m) CCK8 assay was used to detect cell viability of miR-340-5p mimics transfected LOVO and CT26 cells. GAPDH or U6 was used as a load control. Data are presented as the mean ± standard deviation. *P < 0.01 vs. NC inhibitors/sense DNA probe/antisense RNA and #P < 0.01 vs. NC mimics

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