Fig. 5

Exosome-mediated miRNA transport between cells. a Internalization of PKH26-labelled exosomes (red) in GES-1 and SGC-7901 cells were observed through confocal microscopy. Fluorescein phalloidin-FITC (green) was used to stain F-actin, while DAPI (blue) was used to stain nuclei. Scale bar, 20 μm. b Exosomes (green) isolated from BGC-823 cell conditioning medium labeled with GFP-Lv-CD63 (green) and transfected with Cy3-miR-15b-3p (red) were co-cultured with SGC-7901 and GES-1 cells for 24 h, and the fluorescent signals were detected using confocal microscopy. Nuclei are stained blue (DAPI). Scale bar, 20 μm. c The efficiency of exosomes in delivering miR-15b-3p to GES-1 and SGC-7901 cells was analyzed using RT-PCR. Mean ± SEM of the results are presented