Fig. 6

Exosomal transfer of miR-15b-3p enhances malignant transformation in vitro. Proliferation of SGC-7901 and GES-1 cells co-cultured with PBS alone or exosomes containing miR-15b-3p mimics/NC/inhibitor/inhibitor-NC were assessed using colony formation (a), CCK-8 (b) and EdU (c) assays. Scale bar, 100 μm. d Migration and invasion assays of SGC-7901 and GES-1 cell treated with PBS, Exo-NC, Exo-15b-3p-mimics, Exo-inhibitor-NC or Exo-15b-3p-inhibitor. Cells that had migrated and invaded were counted. Representative images are shown. e The SGC-7901 and GES-1 cell apoptosis, in the presence of PBS or exosomes (wrapped with 15b-3p-mimics, inhibitor or their corresponding normal control) were detected using flow cytometry. f miR-15b-3p mimics/NC/inhibitor/inhibitor-NC-enriched exosomes or only PBS was incubated with GES-1 and SGC-7901 cells for 24 h, followed by TUNEL assay. g Western blotting assay of DYNLT1, BAX, BCL-2, Cleaved Caspase-9 and Cleaved Caspase-3 in SGC-7901 and GES-1 with the treatments indicated. The internal control used was β-Actin. Mean ± SEM of three independent experiments are presented