Fig. 2

Increased expression of C3 in GC tissues. a Analysis of C3 expression in unpaired GC tissues and normal tissues in TCGA cohort (P < 0.001, left panel), C3 expression in paired GC and adjacent normal tissues (n = 37) in TCGA cohort (P < 0.001, middle panel), with C5 expression in the paired cohort (P = 0.546, right panel); b C3 expression in Gastric tissues according to Oncomine dataset (P = 2.49E-5; Reporter ID: 217767_at); c The protein levels of complement C3 and its effectors detected with western blot method in GC tissues and respective adjacent normal tissues (left panel; n = 3, left panel), with relative protein levels of C3 and C3a (right panel; n = 106, P < 0.001 vs normal tissues); Deposition of C3, C3a, C5a and the presence of CR1 and factor B in GC tissues were measured with IHC (d) and IFC (e) staining methods, with normal tissues utilized as control. Representative images of n = 5 independent experiments