Fig. 7
Restored AR expression can restore effects of SIRT7 on prostate cancer cells proliferation, autophagy and aggressiveness. a SIRT7 knockdown by shRNA and AR overexpression by plasmids in 22RV1 cells were validated by western blotting. b and c Colony formation assay on 22RV1 cells shows that AR upregulation reversed the effect of SIRT7 depletion on prostate cell proliferation. d CCK8 assay shows that AR upregulation reversed the effect of SIRT7 depletion on prostate cell proliferation. e 22RV1 cells stably expressing the mRFP–GFP–LC3 protein were treated for 3 days with vehicle (ethanol) or DHT. Autophagosomes (yellow) and autolysosomes (red) were covisualized and examined by immunofluorescence microscopy. f Mean number of puncta of each cells (n = 16 cells) was analyzed and plotted. g and h Transwell migration and Matrigel invasion assays in 22RV1 cells show that AR upregulation reversed the effect of SIRT7 depletion on prostate cancer cell migration and invasion. Each assay was performed in triplicate and the data are shown as the means ± SD. P-values were calculated by t-test (*P < 0.05; **P < 0.01; ***P < 0.001)