Fig. 3
A2aR-knockdown enhances proliferation potency of MSLN-CAR T cell. a, b 2 × 105 effector cells including PKH26-Labeled MSLN-CAR T, A2aR.KD1-MSLN.CAR T, A2aR.KD3-MSLN.CAR T, and also Mock T and Un-T as control cells were co-incubated with mitomycin C treated-Hela cell as mesothelin positive target cell at a 1:1 ratio for 72 h in the absence and presence of 1 μM NECA. Anti-CD3 staining was used to distinguish T cells from target cells. PKH dilution was used as a measure of cell proliferation. a Histograms display the percentage of divided effector cells. b Bar graphs show the average percent of proliferated effector cells in different conditions. Data are presented as mean ± SD from a representative experiment (n = 3). MSLN-CAR: fully human anti mesothelin CAR; A2aR-KD-MSLN-CARs: A2aR-knocked down anti mesothelin-CAR; NECA: 5′-(Nethylcarboxamido) adenosine; SD: standard deviation. (***P < 0.001)