Fig. 4
A2aR knockdown prevents inhibition of MSLN-CAR T cell cytotoxicity in the presence of A2aR agonist. a Representative flowcytometry histograms show the cytotoxic capacity of MSLN-CAR, A2aR.KD1 and KD3.MSLN-CAR T cell and two controls (Un-T cells and Mock T cells from one donor). 5 × 104 effector cells were mixed with PKH26-labeled HeLa cells as the target at 4 ratios (effector: target) including 1:1, 5:1, 10:1 and 20:1 in the absence or presence of 1 μM NECA A2aR agonist. b Line plots display average percent of dead target cells in three independent experiments. MSLN-CAR: fully human anti mesothelin CAR; A2aR-KD-MSLN-CARs: A2aR-knocked down anti mesothelin-CAR; NECA: 5′-(Nethylcarboxamido) adenosine; SD: standard deviation. (**P < 0.01, ***P < 0.001)