Fig. 3

CLDN6 promotes HIF-1α degradation via the VHL/PHD-independent pathway. a RT-PCR indicated that CLDN6 overexpression had no significant effect on HIF-1α mRNA expression. b Evaluation of the HIF-1α degradation rate after treating hypoxia cells with CHX for 0, 30, 60 or 120 min. c Cells were treated with MG-132 to assess whether HIF-1α is degraded by the ubiquitin-proteasome pathway in normoxia conditions. d mRNA levels of VHL, PHD1, PHD2 and PHD3 in MDA-MB-231/Vec and MDA-MB-231/CLDN6 cells. e Protein levels of VHL, PHD1, PHD2 and PHD3 in MDA-MB-231/Vec and MDA-MB-231/CLDN6 cells. * p < 0.05