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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: A SUMOylation-dependent HIF-1α/CLDN6 negative feedback mitigates hypoxia-induced breast cancer metastasis

Fig. 7

CLDN6 downregulates SENP1 expression by blocking the nuclear translocation of β-catenin. a ChIP experiment of the combination of c-Jun and β-catenin to the predicted sequences in the SENP1 promoter. b β-catenin mRNA and protein levels in CLDN6-overexpressing MDA-MB-231 cells and CLDN6-KO HBL-100 cells. c β-catenin overexpression rescued SENP1 expression in MDA-MB-231/CLDN6 cells. d Co-IP experiment indicated that CLDN6 and β-catenin combined with each other in MDA-MB-231/CLDN6 cells. e Sequential fractionation to determine the location of CLDN6 and β-catenin. The cytosolic, membrane bound organelles and nuclear fractions are denoted by C, M and N, respectively. f PLA showed the co-localization of CLDN6 and β-catenin in MDA-MB-231/CLDN6 cells. Scale:100um. g WB analysis of β-catenin extracted from the cytoplasm and nucleus. h IF indicating that CLDN6 promotes β-catenin cytoplasm translocation. Scale:50um. * p < 0.05

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