Fig. 2

CUL7 knockdown inhibits cell proliferation and induces cell cycle arrest and cell apoptosis. U87MG and U251 cells transfected with CUL7 siRNA or controls and characterized in the following assays: (a, b) EdU performed 48 h after transfection (scale bar = 100 μm); (c) growth curve based on OD450 using the CCK-8 assay; (d, e) cell cycle profiles determined from PI staining in flow cytometry; (f, g) % apoptosis as determined with Annexin V-FITC antibody and PI staining in flow cytometry. (h) Western blot to detect expression levels of the known cell cycle and apoptosis regulatory factors indicated. GAPDH was used as a loading control. Data are represented as the mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, relative to control.NC: non-silencing siRNA; si-CUL7: siRNAs targeting CUL7