Table 1 The NGS Paradox at a glance: a list of benefits and possible pitfalls

✓ High-throughput technology progress

✓ Great challenge for clinical use

✓ Use and development of targeted drugs

✓ New mutations with no drugs

✓ Broad tumor characterization

✓ Uncertainty in patient outcome

✓ Identification of many diagnostic sub-populations

✓ Many small sub-populations with poor statistical power

✓ Simultaneous gene sequencing

✓ Time consuming

✓ Cost decrease

✓ Not always accessible

✓ Growing number of data

✓ Difficult data interpretation and classification

✓ Many newly discovered variants

✓ Increase of VUS