Fig. 4

Vandetanib inhibits the viability and cell signaling of RET in EOC cells expressing RET mutants. a Drug sensitivity of RET mutants in the CTG assay. A2780 cells transduced with RET R693H or A750T were seeded in 96-well plates in triplicate and treated with 0, 500, 750, 1000, 2500, or 5000 nM vandetanib at 24 h and 72 h after plating. The inhibition effects of vandetanib are shown in the bar graph and normalized to the WT control. b Vandetanib strongly inhibits the phosphorylation of RET and ERK in A2780 cells with RET mutations. A2780 cells expressing either RET R693H or A750T mutant were treated with 500 nM vandetanib for 4 h before harvesting, and the western blotting results are shown in (b). c and d Bar graphs showing quantification of western blotting bands in (b), normalized to WT control. The results represent three repeated experiments *, P < 0.05 compared to WT. **, P < 0.01 compared to WT