Fig. 2

RUNX1 suppresses the growth, migration, invasion and angiogenesis of NB cells in vitro. a Western blot assays showing the expression of RUNX1 in SH-SY5Y and SK-N-SH cells stably transfected with empty vector (mock), RUNX1, scramble (sh-Scb), sh-RUNX1#1 or RUNX1#2 shRNA. b CCK8 assays depicting the change in cell viability of NB cells stably transfected with RUNX1, sh-RUNX1#1, sh-RUNX1#2, or sh-RUNX1#2 after culture for 96 h. c Representative images (left panel) and quantification (right panel) of soft agar plates indicating anchorage-independent growth of NB cells stably transfected as indicated. d Transwell Matrigel invasion assays of representative images (left panel) and quantification (right panel) for 48 h indicating the invasion capability of NB cells stably transfected as indicated. e Representative images (left panel) and quantification (right panel) of the tube formation of endothelial HUVECs treated with medium preconditioned (for 6 h) with NB cells stably transfected as indicated . *P < 0.05 vs. mock or sh-Scb, **P < 0.01 vs. mock or sh-Scb, ***P < 0.001 vs. mock or sh-Scb. Data are shown as mean ± SEM and representative of three independent experiments in panels (a)–(e). Exact P values are specified in Additional file 2: Table S3