Fig. 5

ETV6 dysexpression affects the in vitro migration, invasion and F-actin cytoskeleton expression and arrangement of HCC cells. a ETV6 were over-expressed in HCCLM3 and HuH7 cells stably transfected with PCDH-EF1-MCS-T2A-Puro-ETV6 vector by Lentivirus infection against puromycin screening. Relative ETV6 protein expression levels were determined by WB using GAPDH as the internal reference. b ETV6 were downregulated in HCCLM3 and HuH7 cells by siETV6 transient transfection interference. Relative protein levels were determined by WB using GAPDH as the internal reference. Transwell assays indicated ETV6 overexpression c enhanced and ETV6 knockdown d decreased the in vitro migration and invasion capacities of HCCLM3 and HuH7 cells. e FITC-phalloidin assays indicated ETV6 overexpression or downregulation increased or decreased cytoskeleton F-actin protein expression level and filament arrangement of HCCLM3 and HuH7 cells