Fig. 1

Metastatic CRC cell-derived exosomes were transferred to primary CRC cells and increased malignant traits of primary CRC cells. a The size of particles population ranged from 30 to 150 nm was confirmed by Zetasizer Nano analysis. b Electron micrograph images of purified exosomes from SW620 and LoVo cells were presented. c The symbolic markers of exsomes were detected by western blot from the supernatant and exosomes. d PKH67 staining was used to definite the internalization of exosomes in recipient cells. e CCK8 assays were carried out to determine the viability of treated CRC cell lines at 0, 24, 48, 72, and 96 h. f Colony formation assays were used to measure the proliferation of treated CRC cell lines. g Wound healing assay was carried out to show the migratory ability of treated CRC cells. h The invasion of differential treated CRC cells was verified by transwell assay. i Liver metastasis was conducted using SW480 cells with or without treatment of Exo-SW620. H&E staining was used to observe the morphological changes. j The lung metastatic model was established by SW480 cells treated or nontreated by Exo-SW620. H&E staining had been used as evaluation methods. Data were the means ± SD of triplicate determinants (*P < 0.05, **P < 0.01)