Fig. 4

MALAT1 and exosomal MALAT1 were involved in clinical CRC progression (a) MALAT1 was overexpressed in CRC tissues than that in corresponding nontumor tissues and overexpression of MALAT1 was correlated with CRC metastasis. b The Oncomine boxed plot showed increased MALAT1 expression in colon adenocarcinoma (COAD) tissues using GEO database (GSE5206). c Kaplan–Meier survival analysis was illustrated on the basis of MALAT1 level using TCGA database. d FUT4 expression in CRC tissues was determined and the correlation between FUT4 expression and CRC metastasis was shown. e A positive correlation between MALAT1 and FUT4 was determined by Spearman’s correlation analysis. f Enhanced MALAT1 promoted FUT4 expression while treatment with miR-26a/26b mimic or FUT4 inhibitor reversed the stimulation. g CCK8 assay was carried out to measure the viability of treated SW480 cells. h The proliferative activity was measured by colony formation assay in SW480 with the treatment of MALAT1 mimic, miR-26a/26b mimic or FUT4 inhibitor. i Enhanced MALAT1 promoted the migratory ability while treatment with miR-26a/26b mimic or FUT4 inhibitor attenuated the enhancement effect. j Exosomes derived from siMALAT1-SW620 and siMALAT1-LoVo cells showed decreased effect on the migration and invasion in SW480 and HCT-8 cells compared with control. k Liver model was conducted in SW480 cells treated with Exo-siRNA-SW620 or Exo-siMALAT1-SW620. H&E staining was performed to evaluate the morphological changes. l Lung metastasis was conducted using SW480 cells treated with Exo-siRNA-SW620 or Exo-siMALAT1-SW620. H&E staining had been used as evaluation methods. m The xenograft model was established to show the carcinogenicity of SW480 and SW480 + Exo-SW620, SW480 + Exo-siRNA-SW620 and SW480 + Exo-siFUT8-SW620, the tumor growth curves were showed to trace the effect of exosomes on CRC progression. Data were means ± SD of three independent assays (*P < 0.05, **P < 0.01)