Fig. 3

Formo inhibits EGFR signaling. a, Formo inhibits EGFR signaling in NSCLC cells. HCC827, H3255, H1975, and A549 cells were treated with Formo and Osi, the whole-cell lysates (WCE) were subjected to IB analysis with the antibodies as indicated. β-actin serves as a loading control. b, Formo inhibits EGFR signaling in Ba/F3 cells. EGFR WT or EGFR activating mutants expressing Ba/F3 cells were starved overnight with 0.1% FBS containing cell culture medium. Cells were pre-treated with Formo or Osi for 2 h, and stimulated with 50 ng EGF for 15 min, WCE was prepared and subjected to IB analysis. β-actin serves as a loading control