Fig. 1

NCK1-AS1 is highly expressed in PC tissues. a heatmap for top 10 highly-expressed lncRNAs in the glioma microarray GSE50161, in which the abscissa refers to the sample numbers while the ordinate refers to the differentially expressed lncRNAs; the histogram in upper right corner is the color scale, in which each rectangle indicates the expression for each sample; b NCK1-AS1 expression in glioma microarray GSE35493; c NCK1-AS1 expression in TCGA-GBM predicted on GEPIA (http://gepia.cancer-pku.cn/) (T, tumor; N, normal); d NCK1-AS1 expression in glioma and normal brain tissue samples measured using RT-qPCR, * compared to the Normal tissues, p < 0.05; E, NCK1-AS1 expression in glioma cell lines U251, SHG-441, U87 and T98 and in human glial cell line HEB measured using RT-qPCR, * compared to the HEB cells, p < 0.05; F, NCK1-AS1 expression in brain tissues and glioma tissues validated using RNA-ISH assay, * compared to the HEB cells, p < 0.05. Measurement data were measured using mean ± SD; difference between two groups was measured using the unpaired t test, while differences among multiple groups were analyzed using one-way ANOVA