Skip to main content

Table 2 Primer sequences for RT-qPCR

From: Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/β-catenin axis

GenePrimer sequence
NCK1-AS1F: 5′-GCCGCAGGAGAGACTTAACA-3′
R: 5′-CCTTCGGCTGGGATGACATT-3′
miR-138-2-3pF: 5′-GCGACCCGATTGTCCATAAG-3′
R: 5′-AGCAAGCCTGTCTGATGTGA-3′
TRIM24F: 5′-CATATGCAGCAACAGCAACCG-3′
R: 5′-GAAAGCCATCTGTAGGGGGT-3′
β-cateninF: 5′-CATATGCAGCAACAGCAACCG-3’
R: 5′-GAAAGCCATCTGTAGGGGGT-3’
U6F: 5′-CTCGCTTCGGCAGCACA-3’
R: 5′-AACGCTTCACGAATTTGCGT-3’
GAPDHF: 5′-GAAGACGGGCGGAGAGAAAC-3’
R: 5′-CCATGGTGTCTGAGCGATGT-3’
  1. Note: RT-qPCR reverse transcription quantitative polymerase chain reaction, miR microRNA, TRIM24 tripartite motif containing 24, GAPDH glyceraldehyde-3-phosphate dehydrogenase