Fig. 1
From: ULK1 inhibition as a targeted therapeutic strategy for FLT3-ITD-mutated acute myeloid leukemia
ULK1 inhibition promotes apoptosis in FLT3-ITD AML cell lines. a–e FLT3-ITD AML cell lines (MOLM-13, MV4;11), FLT3-WT AML cell lines (HL60, U937), and FLT3-ITD-inducible U937 cell lines (U937/FLT3-ITD #12, #15) were treated with various concentration of ULK1 inhibitors (MRT 68921; 2.5 μM, SBI-0206965; 5 μM) for 48 h. a, b The fraction of apoptotic cells in FLT3-ITD and FLT3-WT AML cell lines treated with the ULK1 inhibitors was analyzed by flow cytometry based on Annexin-V/PI exclusion. c Cell lysates were subjected to western blotting for ULK1 expression before and after treatment of the cell lines with the ULK1 inhibitors. α-Tubulin was used as a loading control. d, e The fraction of apoptotic cells in U937 AML and U937/FLT3-ITD #12, #15 cell lines treated with the ULK1 inhibitors was analyzed by flow cytometry based on Annexin-V/PI exclusion