Fig. 7

MSI2a inhibited TNBC progression in a TP53INP1-mediated manner. a Wound-healing assays. Representative images and quantification of the assay results show the wound-healing capacity of MSI2a-overexpressing MDA-MB-231 cells transfected with siTP53INP1 or MSI2a-knockdown Hs-578T cells transfected with TP53INP1 plasmids, respectively. Scale bar, 200 μm. b Representative images and quantification of the number of migrated cells of MDA-MB-231 cells coexpressing empty vector or MSI2a and control siRNA or siTP53INP1 as well as Hs-578T cells coexpressing control siRNA or siMSI2a and empty vector or TP53INP1. Scale bar, 100 μm. c MSI2a-overexpressing MDA-MB-231 cells and MSI2a-knockdown BT20 cells were analyzed by Western blot with anti-MSI2, anti-TP53INP1, anti-P73, anti-DUSP10, anti-ERK, and anti-p-ERK antibodies. Vinculin was used as a control. d MDA-MB-231 cells coexpressing empty vector or MSI2a and control siRNA or siTP53INP1 were analyzed by Western blot with anti-MSI2a, anti-TP53INP1, anti-ERK, and anti-p-ERK antibodies. Vinculin was used as a control. *p < 0.05