Fig. 4

ABCA8 blocks EMT in HCC via modulating the ERK/ZEB1 axis. a and b Protein levels and mRNA levels of EMT markers measured by western blot and qPCR. c Representative immunofluorescence images of E-cadherin and vimentin expression in indicated HCC cell lines, Scale bars: 200× = 50 μm. d The expression of Slug, Snail, Twist, ZEB1, and ZEB2 were measured by western blot after ABCA8 overexpression or silencing. e Protein levels of ERK, p-ERK, TGF-β, and p-AKT were analyzed by western blot after ABCA8 overexpression or silencing. Data are means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001