Fig. 3

Circ_0008532 acts as a sponge for miR-155-5p/miR-330-5p. a MiR-155-5p and miR-330-5p were pulled down with anti-ago2 antibody more effectively in the circ_0008532 knock-down group than in the control group. b RNA-FISH revealed that circ_0008532 and miR-155-5p/miR-330-5p colocalizes in the cytoplasm in EJ cells. Nuclei were stained blue with DAPI. Circ_0008532 was stained red with cy3. Locked nucleic acid miR-155-5p/miR-330-5p probes were labeled with Dig. c Schematic showing the predicted binding sites for circ_0008532 and miR-155-5p/miR-330-5p, and mutation of potential miRNAs-binding sequence in circ_0008532 (left). Luciferase activity in bladder cancer cells cotransfected with a luciferase reporter containing either circ_0008532-wt or circ_0008532-mut and miR-155-5p/miR-330-5p mimics or inhibitors (right). d Levels of miR-155-5p/miR-330-5p were downregulated in bladder cancer cells. e Correlation of miR-155-5p/miR-330-5p and circ_0008532 expression in 45 BC tissues. Bar graphs show the statistical analysis of three independent experiments (* P < 0.05)