Fig. 1

Overexpression of survivin is required for maintaining of tumorigenic properties of oral squamous cell carcinoma (OSCC) cells. a Left, the expression of survivin in five representative OSCC tissues was examined by IB analysis. A total of 35 cases of paired OSCC tissue and adjacent non-tumor tissue were subjected to IB analysis. Right, relative survivin expression was determined by quantification analysis. N, adjacent non-tumor tissue, T, tumor tissue. b The expression of survivin in immortalized oral epithelial cell and OSCC cells. c Knockout of survivin decreased cell viability in CAL27 (left) and SCC25 (right) cells. d Knockout of survivin inhibited colony formation of CAL27 (top) and SCC25 (bottom) cells. Scale bar, 200 μm. e-g The tumor volume (e), the image of tumor mass (f), and tumor weight (g) of CAL27-derived xenograft tumors with survivin knockout or not. h-j The tumor volume (h), image of tumor mass (i), and tumor weight (j) of SCC25-derived xenograft tumors with survivin knockout or not. ***p < 0.001. For f and i, Scale bar, 1 cm