Fig. 6

Xanthohumol suppresses in vivo xenograft tumor growth. a-c, Xanthohumol inhibited CAL27-derived xenograft tumor growth. The tumor volume (a), the image of tumor mass (b), and tumor weight (c) of vehicle- and xanthohumol-treated tumors. d-f Xanthohumol inhibited SCC25-derived xenograft tumor growth. The tumor volume (d), image of tumor mass (e), and tumor weight (f) of vehicle- and xanthohumol-treated tumors. For b and e, Scale bar, 1 cm. g IHC staining analysis of Ki67, p-Akt, and survivin in SCC25-derived xenograft tumors. Scale bar, 25 μm. h and i The body weights of CAL27 (h) and SCC25 (i) tumor-bearing mice with vehicle or xanthohumol treatment. j Blood analysis of mice with vehicle or xanthohumol treatment. **p < 0.01, ***p < 0.001