Fig. 2

The effect of circ-001971 on CRC cell invasion and HUVEC tube formation (a) The expression of circ-001971 in five CRC cell lines, HT29, HCT116, LoVo, SW480, and SW620, and a normal cell line was examined using real-time PCR. b The knockdown of circ-001971 was achieved in HCT116 and SW620 cells by transfection with si-circ-001971#1 or si-circ-001971#2, as confirmed using real-time PCR. (C-E) HCT116 and SW620 cells were transfected with si-circ-001971 and examined for DNA synthesis (c) and cell proliferation (d-e). f-g HCT116 and SW620 cells were transfected with si-circ-001971 and examined for cell invasion using Transwell assays. h-i We collected conditioned medium from circ-001971-knockdown or control SW620 cells (si-circ-001971 CM and si-NC CM), cultured HUVECs in these two CM samples, and examined for the tube formation capacity. The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to FHC cells or the si-NC group