Fig. 3
DCZ3301 caused M phase arrest in BTZ-resistant cells. (a) NCI-H929R treated with DCZ3301 with indicated concentrations (the upper panel) and for the indicated time (lower panel) and cell cycle arrest analyzed using PI staining and flow cytometry. (b) The percentage of M phase arrest induced by DCZ3301 was tested by staining with Alexa Fluor®488-conjugated anti-phospho (Ser10)-Histone H3 polyclonal antibody and flow cytometry. Quantification of M phase cell arrest induced by DCZ3301 is shown on the right panel. Data represent the mean of three independent experiments. *p < 0.05 (c) Western blot showing the expression of G2/M phase-related proteins in BTZ-resistant MM cells treated with DCZ3301