Fig. 6

Gli2 completely rescued circ-STAT3 depletion mediated effects on HB cells. a qRT-PCR analysis of Gli2 expression in HB cells transfected with pcDNA3.1-Gli2. Student’s t-test. b-c EdU (scale bar: 100 μm) and colony formation assay detected cell proliferation ability in sh-NC, sh-circ-STAT3#2 and sh-circ-STAT3#2 + pcDNA3.1/Gli2 group. One-way ANOVA. d-e Transwell (scale bar: 200 μm) and wound healing assay (scale bar: 200 μm) examined influence of Gli2 overexpression on circ-STAT3 depletion-mediated invasion and migration ability of HB cells. One-way ANOVA. f-h Sphere formation assay (scale bar: 200 μm), qRT-PCR analysis and western blot analysis demonstrated cell stemness characteristic in sh-NC, sh-circ-STAT3#2 and sh-circ-STAT3#2 + pcDNA3.1/Gli2 group. One-way ANOVA. ^**P < 0.01