Fig. 6

LINC00520 acts as a ceRNA to promote EIF5A2 expression. a The binding sites of miR-125b-5p on the 3′-UTR of EIF5A2, and target sequences were mutated. b Luciferase assay of cells transfected with EIF5A2–3′ UTR-WT or EIF5A2–3′ UTR-MUT reporter together with miR-125b-5p mimic or NC. c Immunoprecipitation (up) of the Ago2/RISC using the Pan-Ago2 antibody in overexpressing miR-125b-5p melanoma cells. IgG was used as a negative control and β-actin was used as an internal control. PCR analysis (down) of miR-125b-5p and EIF5A2 incorporated into RISC in overexpressing miR-125b-5p melanoma cells. d Luciferase assay of cells transfected with EIF5A2–3′ UTR-WT or EIF5A2–3′ UTR-MUT reporter together with LINC00520 siRNA or LINC00520 siRNA plus miR-125b-5p inhibitor. e The expression of EIF5A2 mRNA in melanoma cells transfected with miR-125b-5p mimic, LINC00520 siRNA or LINC00520 siRNA plus miR-125b-5p inhibitor. f Western blots identified EIF5A2 protein expression changes in different groups, GAPDH was used as a control. Data were expressed as the mean ± SD, **P < 0.01, ***P < 0.001