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Table 1 Overview of CRISPR/Cas9 applications or potential use in HCC treatment

From: Description of CRISPR/Cas9 development and its prospect in hepatocellular carcinoma treatment

Category Description of CRISPR/Cas9 function Reference
Interference in HBV Edit coding sequence in cccDNA [53,54,55,56,57]
Edit conserved region [53, 58]
Edit HBsAg protein coding sequence [59]
Participate in combination therapy [60]
Manipulate cancer genome Knock out or insert specific sequence [11, 61]
Create hepatocarcinoma mouse model Knock out specific gene [62]
Edit genome in mouse embryonic cells [63]
Enhance immunotherapy Modify PD-1 of immune cell [64, 65]
Modify CAR-T cell [66]
Induce differentiation of iPSCs [67]
Create potential tool applied in human body Form delivery agent containing target sgRNA [68]
Establish CRISPR/Cas9 libraries Integrate experimental data and prepare for gene screen [12]
  1. Abbreviations: HBV hepatitis B virus, cccDNA covalently closed circular DNA, HBsAg hepatitis B virus surface antigen, PD-1 programmed death 1, CAR-T chimeric antigen receptor-modified T, iPSCs induced pluripotent stem cells, sgRNA single strand RNA