Fig. 3

a Representative images and b relative quantification of morphogenesis assay of HUVEC plated on matrigel exposed for 6 h to the conditioned media (CM) from control or H1299 cells treated with CPTH6 for 24 h at the indicated concentrations. c Analysis of VEGF, VEGFR1 and VEGFR2 mRNA expression in H1299 cells exposed to 20 μM CPTH6 for 24 h. d Quantification of VEGF protein secretion performed by ELISA assay in CM from H1299 control cells or treated as reported in (c). e,f ChIP analysis of (e) histone H3 acetylation (H3acPAN) and (f) Pol II recruitment at three regions (P1-P3) of VEGF promoter of H1299 control cells or treated as in (c). The results represent the average ± SD (b,d) or ± SEM (c,e,f) of three independent experiments. p values were calculated between control and treated conditioned medium (a,b,d) or between control and treated cells (c,e,f). *p < 0.05. **p < 0.01