Fig. 2
Growth-promoting effect of ACYP2 on glioma cells in vitro. a, Western blot analysis was carried out in U251, SF295 and U87 cells to confirm ACYP2 knockdown by two different siRNAs (si-ACYP2–482 and − 540). GAPDH were used as a loading control. b, ACYP2 knockdown significantly inhibited cell proliferation in comparison with the control. c, Knocking down ACYP2 in the indicated cells significantly inhibited colony formation. Representative images showing colony formation in the left panel, and quantitative analysis was shown in the right panel. d, Cell apoptosis in the indicated cells were measured by flow cytometry. The data were presented as mean ± SD (n = 3). e, Ectopic expression of ACYP2 was confirmed by western blot analysis. Tubulin and GAPDH were used as loading controls. f and g, Ectopic expression of ACYP2 significantly promoted glioma cell proliferation and colony formation. The effects of knockdown (h) and ectopic expression (i) of ACYP2 on the proliferation of of primary human glioma cells. The data were presented as mean ± SD (n = 3). Scale bar: 200 μm; *, P < 0.05; **, P < 0.01; ***, P < 0.001