Fig. 4
From: 2,6-DMBQ is a novel mTOR inhibitor that reduces gastric cancer growth in vitro and in vivo
mTOR is a therapeutic target in gastric cancer cells. a Expression mTOR signaling molecules in gastric cancer cells. Cells were seeded and incubated for 48 h and expression of the total or phosphorylated mTOR and p70S6K proteins was analyzed by Western blotting. b Effect of mTOR knockdown on total or phosphorylated mTOR protein. HGC27 gastric cancer cells stably expressing knockdown mTOR or Control were established. The expression of total or phosphorylated mTOR was determined by Western blotting. For a and b, similar results were observed from 3 independent experiments and band density was measured using the Image J (NIH) software program. c, d Effect of mTOR knockdown on growth of gastric cancer cells. Cells were seeded and incubated for 48 h or 2 weeks and cell growth was determined by (c) MTT assay or (d) soft agar assay. For e, colonies were counted using a microscope and the Image-Pro PLUS (v.6) computer software program. For d and e, data are shown as means ± S.D. of triplicate values from 3 independent experiments. The asterisk (*) indicates a significant difference (p < 0.05)