Fig. 1

MMP2-targeted polypeptide JP3 inhibits growth and metastasis of GC in vivo. a The screening flowchart of JWA candidate peptides in nude mice. b The name and amino acid sequence of six fragments. c The ratios of mouse xenograft tumor weight/mice weight from screening model by intratumor injection. d The inhibition rates of tumor growth from screening model by intraperitoneal injection. e The expression levels of MMP2, CD31 and PCNA protein were analyzed using Western blotting in tumor tissues from nude mice bearing GC. f-g Sections of tumors were stained with anti-Ki-67 and anti-CD31 antibodies by IHC (scale bars = 50 μm). h-i The luciferase signals in the mice were detected and photographed using an IVIS in vivo image system. j-k The representative HE staining sections of lung (scale bars = 2 mm). l-m Sections of lung were stained with anti-CD31 antibodies by IHC (scale bars = 50 μm). The data are presented as the means ± SEM, ns: no significance, *P < 0.05, **P < 0.01