Fig. 1

The expression of H19 is upregulated in metastatic CRC and associated with poor prognosis. a Left. Heat-maps of top 10 lncRNAs upregulated or downregulated between tumor and matched adjacent normal samples form GSE50760. Right. Heat-maps of lncRNAs changed most substantially between primary tumors and matched metastatic tissues. b The expression levels of H19 in matched normal colonic epithelium, primary CRC and metastatic liver lesions from GSE50760. Wilcoxon signed-rank test. c Analysis of H19 expression by qRT-PCR in tumors and matched adjacent colonic epithelium tissues of 60 CRC patients from the first affiliated hospital of Sun Yat-sen university. The gene expression is normalized to GAPDH. Student’s t-test. d Analysis of H19 expression in primary tumors between patients with (n = 11) and without metastasis(n = 49) by qRT-PCR. Student’s t-test. e Analysis of H19 expression between paired primary tumors and liver metastasis tissues (n = 11) by qRT-PCR. Student’s t-test. f High H19 expression in CRC correlated with decreased overall survival by Kaplan-Meier survival analysis. The median expression of H19 was used as cut-off. Log-rank test. *P < 0.05, **P < 0.01, ***P < 0.001