Fig. 5

H19 induced migration and invasion in colorectal cancer through hnRNPA2B1 a HnRNPA2B1 knockdown blocked the migration and invasion of HCT116 cells. b HnRNPA2B1 knockdown decreased the migration and invasion of SW480 cells. c Western blot analyses of EMT markers (Snail, E-cadherin and N-cadherin) after silencing of hnRNPA2B1in HCT116 and SW480 cells. d Knockdown of hnRNPA2B1 inhibited H19-induced migration and invasion in HCT116 cells. e Silencing of hnRNPA2B1 decreased H19-induced migration and invasion in SW480 cells. f Ectopic expression of H19 elevated the phosphorylation of ERK in colorectal cancer cells. g Western blot showed altered levels of p-ERK after H19 knockdown in HCT116 and DLD1 cells. h SCH772984 decreased the phosphorylation level of p-ERK, and the expression of Snail in HCT116-H19 and SW480-H19 cells. i HnRNPA2B1 knockdown reversed H19-induced p-ERK and Snail expression. Scales bars = 100um. Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001