Fig. 7
ROS/PI3K/Akt/mTOR axis plays a key role in BDH2-induced apoptosis and autophagy. a Levels of p-AktSer473 and p-mTORSer2448 were examined by western blotting in BDH2-overexpressing and vector control cells. b, c Protein expression levels of p-AktSer473, p-mTORSer2448, LC3-II, p62, cleaved caspase 3, and PARP with or without 740Y-P treatment were measured by western blotting. d Viabilities of SGC7901 and BGC823 cells were detected by CCK-8 assays. e Percentages of apoptotic cells were examined by ANXA5 and PI staining. f–i Detection of autophagic flux by the GFP-mRFP-LC3 lentivirus in BDH2-overexpressing cells. Scale bars, 20 μm. j p-Akt and p-mTOR expression in xenografted tumours was examined by IHC staining. Scale bars, 50 μm. Results are presented as means ± S.D. (n = 3); *p < 0.05, **p < 0.01 compared with vector; ##p < 0.01 compared with BDH2 transfected cells treated with 740Y-P