Fig. 3

CYT997 triggered autophagy in SGC-7901 and MKN45 cells. a After transient transfection with the GFP-fused LC3 plasmid, SGC-7901 and MKN45 cells were treated with CYT997 for 24 h. Cells were incubated with LysoTracker Red DND-99. The distribution of GFP-LC3B expression and LysoTracker Red was visualized by confocal microscopy. Scale bars = 7.5 μm. Results were validated by three separate experiments. b Expression of LC3B and Beclin-1 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997. c SGC-7901 and MKN45 cells were treated with CYT997 alone or in combination with 3-MA. Cell apoptosis was detected by flow cytometry. **p < 0.01.d Expression of cleaved PARP, PARP, cleaved caspase 3 and caspase 3 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997 alone or in combination with 3-MA