Fig. 1
Silencing of SRSF10 affected the proliferation, migration and tube formation of GECs. a The relative protein expression of SRSF10 in NBTs and LGGTs, HGGTs were evaluated by Western-blot assay. GAPDH was used as an endogenous control. Data represent mean ± SD (n = 5, each group; **P < 0.01). b The relative protein expression of SRSF10 in AECs and GECs were evaluated by Western-blot assay. GAPDH was used as an endogenous control. Data represent mean ± SD (n = 3, each group; **P < 0.01). c The effect of SRSF10 on the viability of GECs was determined by CCK-8 assay. Data represent mean ± SD (n = 3, each group; **P < 0.01). d The effect of SRSF10 on the migration of GECs was assessed by Transwell assay. Data represent mean ± SD (n = 3, each group; **P < 0.01). Scale bar represents 30 μm. e The effect of SRSF10 on the tube formation of GECs was evaluated by Matrigel tube formation assay (Black arrow, tube structures and grey arrow, tube branches). Data represent mean ± SD (n = 3, each group; **P < 0.01). Scale bar represents 30 μm. f The effect of SRSF10 on the expression of MMP2. Data represent mean ± SD (n = 3, each group; **P < 0.01). g The effect of SRSF10 on the expression of VEGFA. Data represent mean ± SD (n = 3, each group; **P < 0.01). h The activity of MMP2 in the GECs after downregulation of SRSF10 was detected by gelatin zymography. Data represent mean ± SD (n = 3, each group; *P < 0.05)