Fig. 5

Circ-ATXN1 facilitated angiogenesis of GECs via binding to miR-526b-3p. a The putative miR-526b-3p binding site in circ-ATXN1 (circ-ATXN1-Wt) and the designated mutant sequence (circ-ATXN1-Mut) were illustrated. b Luciferase reporter assay of HEK293T cells co-transfected with circ-ATXN1-Wt or circ-ATXN1-Mut and miR-526b-3p or miR-526b-3p-NC. Data represent mean ± SD (n = 3, each group; **P < 0.01). c-d The interaction between circ-ATXN1 and miR-526b-3p was determined by RIP assay. Circ-ATXN1 expression and miR-526b-3p enrichment were measured using real-time qPCR. Data represent mean ± SD (n = 3, each group; **P < 0.01, ^##P < 0.01). e The co-effects of circ-ATXN1 and miR-526b-3p on the viability of GECs were evaluated by CCK-8 assay. f The co-effects of circ-ATXN1 and miR-526b-3p on the migration of GECs were evaluated by Transwell assay. g The co-effects of circ-ATXN1 and miR-526b-3p on the tube formation of GECs were evaluated by Matrigel tube formation assay (Black arrow, tube structures; Grey arrow, tube branches). Data are presented as the mean ± SD (n = 3, each group; **P < 0.01 vs sh-circ-ATXN1-NC + pre-miR-526b-3p group. Scale bar represents 30 μm). h-i The co-effects of circ-ATXN1 and miR-526b-3p on the expression of MMP2 and VEGFA. Data represent mean ± SD (n = 3, each group; **P < 0.01). j The co-effects of circ-ATXN1 and miR-526b-3p on the activity of MMP2. Data represent mean ± SD (n = 3, each group; **P < 0.01)