Fig. 4

RBPj deficiency in SM22-MCs results in disordered tumor vasculature with poor function. a RBPj^∆SM22 and Ctrl mice were inoculated with LLC cells for 21 days. Tumor sections were stained with CD31 and CD31 plus α-SMA or SM22α. Tumor vessel density and vMC coverage were quantitatively compared (n = 5). b Vessel perfusion was determined by injection of Dextran-2MD, followed by immunostaining. The percentages of perfused vessels were compared between the RBPj^∆SM22 and Ctrl mice (n = 5). c Tumor sections were immunostained with CD31. The intact vessels, which were determined by lumens with continuous CD31⁺ ECs, were determined and compared (n = 5). d RBPj^∆SM22 and Ctrl mice were inoculated with LLC cells. The CD31⁺CD45⁻ ECs in blood were determined by FACS on day 21 and compared between the RBPj^∆SM22 and Ctrl mice (n = 5). e Tumor sections were immunostained with CD31 and SM22α. The distance between SM22-MCs and CD31⁺ ECs was determined and quantitatively compared between the RBPj^∆SM22 and Ctrl mice. Bars = means ± SD. *, P < 0.05 and n.s, not significant