Fig. 4

MK5-AS1 recruited RBM4/eIF4A1. a. RNA FISH analysis of the location of MK5-AS1 (red) in the cytoplasm of HCT116 cells. Original magnifications, × 400 for A. b. RNA pull-down assay showed that MK5-AS1 could not retrieve MK5 from HCT116 cells lysates. c, d. Analysis of the interaction propensities between MK5-AS1 and RBM4 by public databases catRAPID. e. RNA pull-down assays followed by immunoblotting showed that MK5-AS1 bound RBM4 and eIF4A1 in HCT116 cells. f. RIP experiments for RBM4 and eIF4A1 were performed and the coprecipitated RNA was subjected to qPCR for MK5-AS1. g. Coimmunoprecipitation was used to identify interaction between RBM4 and eIF4A1 in HCT116 cells. Data were shown as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001