Fig. 6

MK5-AS1 negatively regulated let-7f-1-3p expression. a. The possible binding sites among MK5-AS1 and microRNAs were predicted by DIANA Tools and miRcode. b. The expressions of miR-1284 and let-7f-1-3p were detected by qPCR in HCT116 and SW620 cells after intervening MK5-AS1, respectively. c. Let-7f-1-3p was downregulated in 42 pairs of CRC tissues compared with normal tissues. d. Correlation analysis of the expression of MK5-AS1 and let-7f-1-3p in 42 pairs of CRC tissues. e. Upper panel, the potential binding sites between MK5-AS1 and let-7f-1-3p. Lower panel, the luciferase reporter plasmids containing wild type (WT) or mutant (MUT) MK5-AS1 were cotransfected into HCT116 cells with let-7f-1-3p. f, g. CCK8 assays and colony formations were used to determine the proliferation of HCT116 and SW620 after transfection. The data represented the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001