Fig. 5From: CircTADA2A suppresses the progression of colorectal cancer via miR-374a-3p/KLF14 axisCircTADA2A mediates the inhibition in cell cycle and aerobic glycolysis and the acceleration in the apoptosis of CRC cells through targeting miR-374a-3p. a qRT-PCR was carried out to detect the level of circTADA2A in LoVo and HCT116 cells transfected with vector or circTADA2A. b qRT-PCR was employed to measure the enrichment of miR-374a-3p in LoVo and HCT116 cells transfected with miR-con or miR-374a-3p. c-p CRC cells were transfected with vector, circTADA2A, circTADA2A + miR-con or circTADA2A + miR-374a-3p, respectively. c and d The cell cycle of CRC cells was evaluated by flow cytometry. e and f The apoptosis of CRC cells was assessed by flow cytometry. g-j The ECAR and OCR of CRC cells were analyzed by Seahorse XFe 96 Extracellular Flux Analyzer. k-p The glucose uptake, lactate production and ATP level in CRC cells were measured by Glucose Uptake Colorimetric Assay kit, Lactate Assay Kit II and ATP Colorimetric Assay kit, respectively. **P < 0.01, ***P < 0.001Back to article page