Fig. 6
From: CircTADA2A suppresses the progression of colorectal cancer via miR-374a-3p/KLF14 axis
MiR-374a-3p could bind to KLF14 in CRC cells. a DIANA TOOL predicted that there existed binding sites between miR-374a-3p and KLF14 mRNA. b and c The luciferase activity was detected in CRC cells co-transfected with KLF14-WT or KLF14-MUT and miR-con or miR-374a-3p. d and e The target relationship between miR-374a-3p and KLF14 was also validated by RIP assay. f The mRNA level of KLF14 in CRC tissues (n = 70) and adjacent non-tumor tissues (n = 70) was determined by qRT-PCR. g The protein expression of KLF14 was examined in CRC tissues (n = 6) and matching normal tissues (n = 6) by Western blot assay. h and i) The abundance of KLF14 mRNA and protein was determined in four CRC cell lines and NCM460 cells by qRT-PCR and Western blot assay, respectively. *P < 0.05, **P < 0.01, ***P < 0.001