Fig. 10
IFI6 promotes the growth of xenograft tumors from Eca109 cells. a-b. Representative image of fresh tumor tissues and tumor size quantification results in the xenograft model. Athymic nude mice were inoculated with the indicated Eca109 cells, and the tumor volumes (mm3) were calculated every 5 days during implantation. The data are presented as the means and SDs (n = 5). Statistical significance was determined by two-tailed Student’s t-test. **P < 0.01. c. Representative image (upper) and quantitative results (bottom) of the IHC measuring the abundance of IFI6, ATF3 and NOX4 in indicated tumor tissues derived from xenograft model. Magnification: 40×. Scale bar: 50 μm. Statistical significance was determined by Mann-Whitney test. *P < 0.5. d. Representative immunoblot showing IFI6, ATF3, NOX4 and ROS markers in indicated tumor tissues derived from xenograft model. GAPDH was used as the loading control. e. Tumor size quantification results in the xenograft model. The data are presented as the means and SDs (n = 5). Statistical significance was determined by two-tailed Student’s t-test. **P < 0.01. f. Representative immunoblot showing IFI6 and ROS markers in indicated tumor tissues derived from xenograft model. GAPDH was used as the loading control. g. Tumor size quantification results in the xenograft model. The data are presented as the means and SDs (n = 5). Statistical significance was determined by two-tailed Student’s t-test. **P < 0.01. h. Representative immunoblot showing IFI6, ATF3 and NOX4 in indicated tumor tissues derived from xenograft model. GAPDH was used as the loading control