Fig. 7

IFI6 modulates mitochondrial ROS production and OXPHOS efficiency by regulating mitochondrial supercomplex assembly. a. Mitochondrial proteins extracted from the indicated ESCC cells were solubilized with digitonin and subjected to BN-PAGE followed by immunoblotting. Mitochondrial supercomplexes were first visualized by incubation with antibodies against complex I (CI, NDUFB8), and the membrane was then stripped and reprobed with antibodies against complex III (CIII, RISP). The membrane was then sequentially probed with antibodies against complex IV (CIV, COXIV), complex II (CII, SDHA) and complex V (CV, ATPB). b-c. Quantitative results for experiments shown in a. Data are presented as mean and SD (n = 3). Statistical significance was determined by two-tailed student’s t-test. **P < 0.01. d-e. Representative image (d) and quantitative results (e) of immunoblotting with antibodies against the indicated individual ETC complexes (I through V). GAPDH was used as the loading control. The data are presented as the means and SDs (n = 3). Statistical significance was determined by a two-tailed Student’s t-test