Fig. 9

NSCLC cells with differential circ-CPA4 expressions influenced CD8⁺ T cell expansion and activation in the co-culturing system. a Knock-down of circ-CPA4 promoted cell proliferation in CD8⁺ T cells, while circ-CPA4 overexpression had opposite effects. b The effects of NSCLC cells differential circ-CPA4 expressions on CD8⁺ T cell viability were determined by trypan blue staining assay. c-e FCM was conducted to examine the apoptosis ratio of CD8⁺ T cells in the co-culturing system. f-g Western Blot was performed to detect the expression levels of Bax in CD8⁺ T cells co-cultured with NSCLC cells. h, i Real-Time qPCR was conducted to detect the mRNA levels of inflammatory cytokines (IFN-γ, IL-4 and IL-10) in CD8⁺ T cells. j, k The expression status of IFN-γ, IL-4 and IL-10 in the supernatants were measured by ELISA. Each experiment repeated at least 3 times. *P < 0.05, **P < 0.01, NS means no statistical significance