Fig. 2

COL4A1 knockdown inhibits the proliferation, migration and invasion of HCC cells. a Knockdown efficiency of COL4A1 using shRNA was confirmed by qRT-PCR and western blot in SMMC7721 cells and SK-Hep1 cells. b-d COL4A1 knockdown inhibited cell proliferation (b), migration (c) and invasion(d) by Real-time cell analyzer. e Downregulation of COL4A1 inhibited cell migration by wound healing assay. f COL4A1 knockdown inhibited cell proliferation by colony formation assay. g COL4A1 knockdown suppressed tumor growth in vivo. n = 7/group. Tumor volume was measured, and photographs of tumors were taken. Data are presented as means ± standard deviation. Student t test and Two-way ANOVA followed by Tukey’s multiple comparisons test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001