Fig. 3

COL4A1 overexpression promotes the abilities of cell proliferation, migration and invasion in HCC cells. a CRISPR/Cas9/Synergistic Activation Mediator (SAM) - mediated overexpression of COL4A1 in HepG2 cells and PLC/PRF/5 cells were confirmed by qRT-PCR and western blot. b-d Stable overexpression of COL4A1 promoted cell proliferation (b), migration (c) and invasion (d). Data are presented as means ± standard deviation. Student t test and Two-way ANOVA followed by Tukey’s multiple comparisons test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001